Final answer:
In TLC plate preparation, ensure uniform sample application, avoid excessive solvent use, provide ample spotting area, and dry the plate quickly and properly.
Step-by-step explanation:
When preparing a TLC plate, it is important to pay attention to a few key things to ensure that your results are accurate and reproducible. One should be careful of uniform sample application, which means applying your sample spots evenly and consistently to avoid merging or spreading which can affect the separation process. Excessive solvent use can also be problematic as it might cause the samples to dissolve too much and interfere with the separation. The spotting area must not be limited; ensure there is enough space for the sample to be spread without overcrowding. Quick plate drying is also essential; the plate should be dried properly before being placed in a developing chamber to prevent smearing or unequal migration of the components.