Final answer:
The ACK# is found in the header of a network frame, which signals the next expected packet for reliable communication in protocols like TCP.
Step-by-step explanation:
In networking, the part of a network frame that contains an ACK# (Acknowledgment Number) to inform the receiving end which packet is expected next is located in the header of the frame. Specifically, in the context of Transmission Control Protocol (TCP), the acknowledgment number is part of the TCP header, which is itself encapsulated within the overall network frame structure. This number is crucial for ensuring that data is received in order and that any missing packets are detected and can be retransmitted.