Final answer:
The challenge to achieving the Human Genome Project's goals included designing a PCR reaction, performing millions of separate sequencing reactions, creating a method to determine the DNA sequence, and placing millions of short DNA sequences into the correct order.
Step-by-step explanation:
The challenge to achieving the Human Genome Project's goals included:
- Designing a PCR reaction to sequence an entire chromosome at once
- Performing millions of separate sequencing reactions
- Creating a method to determine the DNA sequence
- Placing millions of short DNA sequences into the correct order
These were all crucial steps in the process of sequencing the human genome. For example, designing a PCR reaction that could sequence an entire chromosome at once allowed for faster and more efficient sequencing. Additionally, creating a method to determine the DNA sequence and placing millions of short DNA sequences into the correct order were essential for accurately mapping the entire genome.