Question

You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not more than 2 pages provide answers for the following questions?

Which tube from the three is the most appropriate to use and why?
a) The third tube, as it contains purified mRNA from pancreatic β-cells during the absorptive state, likely having active proinsulin production.
b) The first tube, since it contains purified mRNA from pancreatic β-cells from a patient in a post-absorptive state, ensuring stable proinsulin levels.
c) The second tube, as it contains purified mRNA from pancreatic α-cells during the absorptive state, potentially indicating a transition phase.
d) None of the tubes are appropriate due to the variability in cell types and states.

Answer 1

The most appropriate tube to use for cloning the gene encoding the proinsulin C-peptide is the third tube, as it contains purified mRNA from pancreatic β-cells during the absorptive state, likely having active proinsulin production.

Step-by-step explanation:

The most appropriate tube to use for cloning the gene encoding the proinsulin C-peptide is the third tube. This tube contains purified mRNA isolated from pancreatic β-cells from a patient during the absorptive state.

During the absorptive state, pancreatic β-cells are likely to have active proinsulin production, making it the ideal source for cloning the proinsulin C-peptide gene.