Question

Design Primers that will amplify the following region of DNA (Primers should be 6 nucleotides each)

ATCGGC TTAGTA GTATAT ACCC
TAGCCG AATCAT CATATA TGGG

Answer 1

Primers for PCR amplification of DNA should be complementary to the target sequence ends. The proposed top primer is 5'-ATGGGG-3' and the bottom primer is 5'-GGCCGA-3', and they bind to their complementary sequences for replication.

Step-by-step explanation:

The task requires designing two primers that are complementary to the specified DNA sequence. Each primer should be 6 nucleotides in length for successful PCR amplification. To design these primers, we take into account the 5' to 3' orientation and identify sequences that can anneal to the 3' ends of the target DNA. Assuming the provided sequence is the top strand of DNA, one primer should be 5'-ATGGGG-3' (top primer) and the other should be made complementary to the bottom strand, which would be the reverse complement of the bottom strand's first six nucleotides, resulting in 5'-GGCCGA-3' (bottom primer).

When conducting the PCR, these primers will bind to their respective complementary sequences on the DNA strand. This allows for the DNA polymerase to extend the primers and replicate the target region. The resulting amplification of the DNA between the primers is critical for various applications such as cloning, sequencing, or analysis. Proper primer design is crucial for the success of the PCR process.