Final answer:
In DNA transcription, the template strand guides the synthesis of the RNA transcript while the coding strand has a sequence nearly identical to the RNA, with U instead of T.
Step-by-step explanation:
The strand of DNA that is used to produce the RNA transcript is called the template strand, while the opposite strand of DNA is called the coding strand or non-template. The RNA transcript is synthesized using the template strand as a guideline, where the nucleotides pair up with the complementary bases on the template strand, with the exception of uracil (U) pairing with adenine (A) instead of thymine (T). The coding strand, also known as the sense strand, has a sequence nearly identical to the RNA molecule except that RNA contains uracil instead of thymine. Transcription, the process of creating the RNA transcript from the DNA template, involves the enzyme RNA polymerase which adds ribonucleotides to the growing mRNA chain in a 5' to 3' direction.