Final answer:
The order of the gene on the chromosome can be determined by analyzing the co-transformation frequency. If the co-transformation frequency is high between two loci, it indicates that those two genes are likely to be located close to each other on the chromosome. By comparing the co-transformation frequencies of different loci, you can establish the order of the genes on the chromosome.
Step-by-step explanation:
The order of the gene on the chromosome can be determined by analyzing the co-transformation frequency. In this experiment, you extracted DNA from a wild-type strain of E. coli and used it to transform a strain that was leu- gal- pro- and thr-. After the initial selection for transformation of the individual loci, you can determine the co-transformation frequency for the other loci to establish the order of the gene on the chromosome.
In the experiment, if the co-transformation frequency is high between two loci, it indicates that those two genes are likely to be located close to each other on the chromosome. On the other hand, if the co-transformation frequency is low, it suggests that the genes are further apart on the chromosome. By comparing the co-transformation frequencies of different loci, you can establish the order of the genes on the chromosome.
For example, if the co-transformation frequency for loci A and B is high, while the co-transformation frequency for loci A and C is low, it suggests that loci A and B are likely to be closer together on the chromosome than loci A and C. By analyzing the co-transformation frequencies for all the loci, you can determine the order of the genes on the chromosome.