Final answer:
Transcription occurs from the 3' to 5' end of the DNA template strand and results in an RNA molecule synthesized from the 5' to 3' end.
Step-by-step explanation:
The direction of transcription is from the 3' to 5' end of the template strand of DNA to the 5' to 3' end of the resulting RNA molecule.
During transcription, the enzyme RNA polymerase reads nucleotides in a 3' to 5' direction on the DNA template strand and assembles the mRNA transcript in a 5' to 3' direction. The mRNA product is complementary to the template strand of DNA, and the only difference is that T nucleotides are replaced with U nucleotides.