Question

Determine the correct sequence of events during the creation of recombinant DNA.

I. Plasmid is cut open with enzymes
II. Target gene is cut out with enzymes
III. Plasmid is inserted into the bacterium
IV. Target gene is bonded to the plasmid with Ligase
A. IV, III, 1, 11
B. IV, I, II, III
C. I, II, III, IV
D. II, I, IV, III

Answer 1

The correct order in gene cloning for creating recombinant DNA is cutting the plasmid, cutting the target gene, joining them with ligase, and then inserting the plasmid into a bacterium. The correct sequence is C. I, II, III, IV.

Step-by-step explanation:

The correct sequence of events during the creation of recombinant DNA is:

Plasmid is cut open with enzymes.

Target gene is cut out with enzymes.

Target gene is bonded to the plasmid with ligase.

Plasmid is inserted into the bacterium.

The correct option is C. I, II, III, IV. This sequence reflects the steps of gene cloning, where a restriction enzyme is used to cut the plasmid DNA and the target gene, which are then joined together with DNA ligase to form recombinant DNA. Finally, the recombinant plasmid is introduced into a bacterium to produce the protein of interest.