Final answer:
Jagendorf's experiments confirmed the role of a proton gradient across the thylakoid membrane in ATP synthesis, through a process called chemiosmosis involving ATP synthase.B. A proton gradient across the thylakoid membrane led to the production of ATP.
Step-by-step explanation:
Jagendorf's experiments confirmed that a proton gradient across the thylakoid membrane led to the production of ATP. This process is known as chemiosmosis, where the buildup of hydrogen ions within the thylakoid lumen creates an electrochemical gradient with the potential energy used to synthesize ATP. The hydrogen ions, or protons (H+), move down this gradient through a protein called ATP synthase, which generates ATP from ADP and an inorganic phosphate (Pi).