Question

What is a disadvantage of using a restriction enzyme such as EcoR1?

1) It doesn't create sticky ends that match to complementary base pairs
2) It is expensive to produce
3) It is difficult to use
4) It has a low efficiency

Answer 1

The disadvantage of using a restriction enzyme like EcoRI is that it can be expensive to produce. EcoRI creates sticky ends for DNA splicing, and is generally efficient and user-friendly for trained individuals. Therefore, The correct option is 1) It doesn't create sticky ends that match to complementary base pairs.

Step-by-step explanation:

A disadvantage of using a restriction enzyme such as EcoRI is that it can be expensive to produce. While EcoRI does indeed create sticky ends that match to complementary base pairs, the cost of producing these enzymes can be a limitation for laboratories with limited budgets. EcoRI, like other restriction enzymes, cuts at a specific recognition site to produce sticky ends that can anneal with complementary DNA fragments. This is essential for creating recombinant DNA molecules. Furthermore, the efficiency of EcoRI is generally high, provided it is used under proper conditions, and it is not particularly difficult to use for someone trained in molecular biology techniques.