Question

In PCR, which of the following temperature ranges is used in the priming process?

1) 40-50°C
2) 50-60°C
3) 60-70°C
4) 70-80°C

Answer 1

In PCR, the priming or annealing process occurs at a temperature range of 50-60°C where primers bind to the DNA template for polymerization.

Step-by-step explanation:

During a Polymerase Chain Reaction (PCR) process, the priming step, also known as the annealing step, involves binding of primers to the single-stranded DNA template. This occurs after the denaturation step where the double-stranded DNA is separated at high temperatures. According to the information provided, the annealing occurs within the range of 50-60°C, which matches with the second option provided in your question. It is essential to have the correct temperature for the primers to bind effectively to the complementary sequences of the DNA template.