Question

Could someone help me to understand the following sentence better? It's from the book A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery. And it's about fluorophors used in FRET-assays, for screening of protease inhibitors.

In contrast to many other FRET dye pairs, both EDANS & DABCYL and Mca/Dnp generate a dynamic range between cleaved an uncleaved substrate, sufficent for the application of such protease assays in HTS and high-throughput compound profiling under initial conditions.

I'm confused about the last part of the quote under initial conditions. What does that mean? the question summarized What is the dynamic range under initial conditions?

Answer 1

The dynamic range refers to the range of signal intensities that can be observed in a FRET assay. In this case, the dynamic range of the fluorophores EDANS & DABCYL and Mca/Dnp is sufficient for their use in protease assays during high-throughput screening under initial conditions.

Step-by-step explanation:

The term "dynamic range" refers to the range of values that a measurement can detect or distinguish. In the context of the sentence you provided, the dynamic range refers to the range of signal intensities that can be observed in a FRET assay when using the fluorophores EDANS & DABCYL and Mca/Dnp. This dynamic range is sufficient for the application of these protease assays in high-throughput screening (HTS) and compound profiling under initial conditions.