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In traditional plant cloning, why do we require two different vectors (plasmids)?

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Final answer:

Two different vectors (plasmids) are required in traditional plant cloning for comparing the effects of the transgene with a control vector.

Step-by-step explanation:

Traditional plant cloning requires two different vectors (plasmids) for a specific purpose. One vector is used to carry the desired DNA fragment, while the other vector serves as a control or reference.

Having two different vectors allows scientists to compare the effects of the transgene (the foreign DNA fragment) with the control vector. This comparison helps to determine if any changes or effects observed in the plant are due to the transgene or other factors.

For example, if the transgene is inserted into one vector and the control vector is empty, any changes observed in the plant can be attributed to the transgene. This allows scientists to study the specific effects of the transgene on plant characteristics.

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