Question

I am looking to purchase Pyruvate Kinase from the Sigma Website, they state the volume in Kilo Units (KU) i.e. 1, 5 or 25 KU. It also states there are 350-600 units / mg protein.

Does this mean one unit is one protein? so 1KU is 1000 units of protein? What is the relationship between the KU and mg? and how can I use this relationship to calculate my concentration in um or mg

Answer 1

KU represents enzyme activity, and 1 KU is equal to 1000 units of protein. The statement '350-600 units / mg protein' means that there are 350 to 600 units of enzyme activity per milligram of protein.

Step-by-step explanation:

The units KU stands for Kilo Units, which represents the enzyme activity. 1 KU is equal to 1000 units of protein. The statement '350-600 units / mg protein' means that there are 350 to 600 units of enzyme activity per milligram of protein.

This relationship between KU and mg allows you to calculate the concentration of the enzyme. To convert the concentration to micromoles (um) or milligrams (mg), you would need to know the total volume of the enzyme solution.