Final answer:
In order to achieve the required sample size of 100 samples for your experiment with 3 treatments and 4 replicates, each replicate should have approximately 8 samples distributed evenly.
Step-by-step explanation:
When calculating the sample size for an experiment, the number of samples needed is typically for each treatment or condition being tested. In your case, you have 3 treatments and 4 replicates, so you would need a total of 12 replicates (3 treatments x 4 replicates). Therefore, in order to meet the required sample size of 100 samples, each replicate should have approximately 8 samples (100 samples ÷ 12 replicates). It is important to distribute the samples evenly among the replicates to ensure accurate results.