Question

I am interested in expressing custom proteins in a Salmonella strain, however I am facing difficulties in finding the appropriate expression vector for it. It seems that most of the resources provide for E. coli expression. Does anyone have any recommendations?

Answer 1

To express custom proteins in a Salmonella strain, you can use vectors like pBR325 or pUCP18.

Step-by-step explanation:

When expressing custom proteins in a Salmonella strain, it can be challenging to find the appropriate expression vector, as most resources provide vectors for E. coli expression. However, there are options available for Salmonella expression vectors. One commonly used vector is pBR325, which is compatible with both E. coli and Salmonella. Another option is the pUCP18 vector, which has been successfully used for Salmonella expression. These vectors can be used to clone the desired genes and express them in a Salmonella strain.