Final answer:
To replace DMSO with an LC-MS-compatible solvent for samples from a well plate, you can use liquid-liquid extraction (LLE) or solid-phase extraction (SPE) techniques. LLE involves using an organic solvent with low miscibility in water, while SPE utilizes LC-MS-compatible sorbents to retain and elute the target compounds.
Step-by-step explanation:
To effectively replace DMSO with an LC-MS-compatible solvent for samples from a well plate, you can consider using liquid-liquid extraction (LLE) or solid-phase extraction (SPE) techniques.
For LLE, you need to select an organic solvent with low miscibility in water. Examples of such solvents include methyl t-butyl ether (MTBE) and ethyl acetate. You can use a mixture of acetonitrile and MTBE as the extraction solvent. The procedure involves adding the extraction solvent to the sample, shaking or vortexing it to facilitate the partitioning of the target compounds into the organic phase, and then separating the organic phase for further analysis.
For SPE, you can use LC-MS-compatible SPE cartridges or plates. These sorbents can retain the target compounds while eluting them with small quantities of an LC-MS-compatible solvent, such as acetonitrile or methanol. The steps involve conditioning the SPE sorbent, loading the sample onto the sorbent, washing off interfering substances, and then eluting the target compounds with the LC-MS-compatible solvent.