Question

I have a specific human protein that was purified from E. coli.

This protein was tagged with MBP and diluted in an elution buffer.
This protein has a tendency to aggregate, and when it does, it can induce the nearest protein to be aggregated too.
We believe that tagging MBP prevents this protein from aggregating, so that when MBP is cut off from the protein, it will easily aggregate.
I want to confirm this. However, I am not sure how to check if the protein is aggregating or not.
I would like to know whether my protein is aggregated, what is the best method to detect protein aggregation?

Answer 1

The best method to detect protein aggregation is through the use of techniques such as gel electrophoresis and size exclusion chromatography.

Step-by-step explanation:

The best method to detect protein aggregation is through the use of techniques such as gel electrophoresis and size exclusion chromatography. Gel electrophoresis separates proteins based on their size and charge, allowing you to visualize any aggregates that may have formed. Size exclusion chromatography separates proteins based on their size, with aggregates being detected by a shift in their elution position compared to monomeric proteins.