Final answer:
To distinguish clones in which the gene is reverse cloned, Southern blotting can be used. This technique involves purifying the cloned DNA, subjecting it to restriction endonuclease digestion, agarose gel electrophoresis, and nylon filter blotting. The smallest DNA fragment containing the gene of interest can be subcloned for further study.
Step-by-step explanation:
In order to distinguish the clones in which the gene is reverse cloned from the others, you can use a technique called Southern blotting. Southern blotting involves purifying the cloned DNA, subjecting it to restriction endonuclease digestion, and separating the digested particles by agarose gel electrophoresis. The separated DNA fragments are then blotted onto a nylon filter and probed with a tagged probe. The smallest DNA fragment containing the gene of interest can be further subcloned in a suitable vector for further study.