Question

How to define the organism by using only FASTQ file with reads?

Answer 1

To define the organism from a FASTQ file, metagenomic analysis is performed where DNA sequences are compared against known genomes, involving steps like quality control, alignment, identification, and validation.

Step-by-step explanation:

To define the organism from which a FASTQ file with reads comes, you typically need to conduct a process known as metagenomic analysis or genomic matching. A FASTQ file contains a large number of DNA sequences (reads) from an organism's genomic material. To identify the organism, these reads can be compared against a database of known genomes. Here is a simplified step-by-step process:

1. Quality Control: Check the sequence quality of the reads in the FASTQ file.
2. Alignment: Align the reads to a reference genome or a database of genomes, such as the NCBI database.
3. Identification: Analyze aligned sequences to match genomic signatures that can identify the species.
4. Validation: Validate the identified organism with additional biological techniques if necessary.

This analysis is typically conducted with specialized bioinformatics tools and software, and it requires a substantial amount of computer processing power and technical expertise in genomic data analysis.