Final answer:
The main differences between the Lenti-Cas9-2A-Blast and lentiCas9-Blast plasmids could be in their genetic components which impact gene expression. Both function as vehicles for CRISPR-based gene editing, utilizing viral vector mechanisms to deliver the necessary gene array for precision gene editing.
Step-by-step explanation:
The differences between dual vector CRISPR/Cas9 lentiviral plasmids Lenti‐Cas9‐2A‐Blast and lentiCas9-Blast are not directly provided, but typically, such differences could involve the arrangement or composition of genetic elements within the plasmids such as promoters, antibiotic resistance genes, or the presence of additional genes (like the 2A peptide sequence in Lenti-Cas9-2A-Blast that allows for multicistronic expression).
In the context of CRISPR/Cas gene arrays, these plasmids would contain necessary components for gene editing including the Cas gene, spacer DNA, and potentially others like leader DNA and tracr gene. These are designed in a way to achieve precision gene editing in various cell types.
Viral vectors and shuttle vectors are two methods used to introduce recombinant DNA into animal cells. A viral vector takes advantage of a virus's ability to infect host cells and deliver genetic material, while a shuttle vector is a plasmid designed to replicate in multiple species of organisms. CRISPR/Cas systems can be delivered into cells using such vectors to conduct gene editing.