Final answer:
The most suitable filtering apparatus for sterilizing ADC is a 0.22 µm PEF filter. Filters clogged likely due to insoluble particles in the ADC solution. HEPA filtration is widely used in hospital settings and biological safety cabinets to remove microbes from the air. option (B)
Step-by-step explanation:
The appropriate filtering apparatus/material for sterilizing a solution containing Albumin, Dextrose, and Catalase (ADC) would likely be a HEPA filtration system or a membrane filter with a pore size adequate for removing bacteria, typically 0.2 µm. In the case of ADC, because catalase is a relatively large protein (232 kDa), a 0.22 µm PEF filter might be necessary to ensure sterilization without clogging. Using a HEPA filter or vacuum filtration apparatus with filters of 0.22 µm or 0.45 µm could lead to clogging due to the presence of large protein molecules (e.g., catalase) or other insoluble particles in the ADC solution. This indicates that option b, the 0.22 µm PEF filter, would be the suitable choice for sterilizing ADC. However, in practice, these filters clogged, likely because the ADC contained insoluble particles that the filter could not process, thus confirming that the correct response to why these filters clogged when attempting to filter ADC is option b: The ADC contained insoluble particles.
HEPA filtration is commonly used in hospital ventilation systems and biological safety cabinets in laboratories to prevent transmission of airborne microbes. Additionally, membrane filtration is an essential technique for removing bacteria from various heat-sensitive solutions used in the laboratory.