Question

Can plasmids be linearized in a sequence-independent manner as described in the text?

A) Yes, by using DNAase I treatment followed by heat inactivation
B) No, plasmid linearization always requires specific sequence recognition
C) Yes, by placing the plasmid in a dialysis bag and applying an electric field
D) Yes, by vortexing the plasmid to cause physical shearing

Answer 1

Plasmids can be linearized in a sequence-independent manner through DNAase I treatment followed by heat inactivation or by physical shearing such as vortexing.

Step-by-step explanation:

Can plasmids be linearized in a sequence-independent manner? Yes, plasmids can indeed be linearized in a sequence-independent manner. Options A and D in the provided choices both describe methods that can linearize plasmids without requiring specific sequence recognition. DNAase I treatment followed by heat inactivation can randomly cleave DNA, thereby linearizing plasmids. However, care must be taken as over-treatment can lead to excessive digestion. Physical shearing, such as vortexing, can also break plasmids into linear forms, though it is less controlled and typically not the preferred method for precise molecular biology work.