Question

Can RNA-specific electrophoresis kits using SYBR Gold be effectively used for sizing and quantification of ssDNA?

a. SYBR Gold RNA kits are suitable for ssDNA sizing and quantification.
b. RNA-specific electrophoresis may not provide accurate results for ssDNA.
c. Buffer and gel properties in RNA kits hinder ssDNA analysis.
d. There is no significant difference between RNA and DNA kits for ssDNA analysis.

Answer 1

RNA-specific electrophoresis kits using SYBR Gold are not suitable for ssDNA sizing and quantification due to their design and optimized properties for RNA analysis.

Step-by-step explanation:

The use of RNA-specific electrophoresis kits with SYBR Gold is not suitable for sizing and quantification of ssDNA. The kits are specifically designed for RNA analysis and may not provide accurate results when used with ssDNA. The buffer and gel properties in RNA kits are optimized for RNA analysis, which can hinder the analysis of ssDNA. It is recommended to use DNA-specific electrophoresis kits for accurate sizing and quantification of ssDNA.