Question

How much of protein B is needed to achieve close to 100% binding with 7mg of protein A on a column?

a. Convert 7mg of protein A to 1.8mg of protein B for optimal binding.
b. Use an excess of protein B to ensure complete binding.
c. The dissociation constant (K_D) is irrelevant in this context.
d. Adjust protein B amount based on the column capacity.

Answer 1

To achieve close to 100% binding with 7mg of protein A on a column, an excess of protein B should be used. The dissociation constant is irrelevant in this context, and the amount of protein B should be adjusted based on the column capacity.

Step-by-step explanation:

To achieve close to 100% binding with 7mg of protein A on a column, you would need to use an excess of protein B. This ensures that all of the protein A is bound to protein B. The dissociation constant (KD) is irrelevant in this context because we are aiming for complete binding. Additionally, you should adjust the amount of protein B based on the capacity of the column to avoid overloading.