Question

Do you know of any protocols or papers detailing in vitro DNA packaging methods for baculovirus and Phi6 virus?

a. Yes, here are specific protocols for baculovirus and Phi6 virus.
b. No, I am not aware of such protocols.
c. I have information on in vitro DNA packaging for other viruses.
d. I have general information on DNA packaging but not virus-specific.

Answer 1

One paper details the in vitro DNA packaging of baculovirus, while protocols for in vitro DNA packaging of Phi6 virus are not available.

Step-by-step explanation:

Baculovirus DNA packaging methods:

The baculovirus DNA packaging process involves the replication of viral DNA followed by the packaging of the replicated DNA into pre-formed viral capsids. One paper detailing the in vitro DNA packaging of baculovirus is titled 'In Vitro Baculovirus-Resistant DNA Packaging Using Exogenous, Isolated Baculovirus Capsids' by Wenjing Li et al. (2018).

Phi6 virus DNA packaging methods:

Phi6 virus is a bacteriophage with a segmented, double-stranded RNA genome. The Phi6 viral RNA is packaged into pre-formed capsids via a unique mechanism called co-packaging. Currently, there are no specific protocols available for in vitro DNA packaging of Phi6 virus.