Question

In MTT assay data processing, what is the function of dividing blank-subtracted values by the mean blank-subtracted value for dose = 0 on each plate?

a. It suppresses plate-to-plate variation.
b. It enhances the accuracy of dose-response modeling.
c. It is not a standard procedure.
d. The purpose is unclear.

Answer 1

Dividing blank-subtracted values by the mean blank-subtracted value for dose = 0 on each plate in MTT assay data processing suppresses plate-to-plate variation.

Step-by-step explanation:

Dividing blank-subtracted values by the mean blank-subtracted value for dose = 0 on each plate in MTT assay data processing serves the purpose of a. suppressing plate-to-plate variation. By normalizing the data, any variations due to plate differences are minimized, allowing for accurate comparison between different plates. This is particularly important when conducting dose-response modeling and comparing the effects of different doses. Therefore, option a is the correct answer.