Final Answers:
1.The purpose of mRNA/cDNA display in biotechnology is a. To generate random protein libraries.
2.Proteins selected for ATP binding also having ATP hydrolysis activity suggests b. It can create enzymes with functional activities.
3.More stringent selection schemes might be used after the initial selection for enzymatic activity to b. To improve initial activity.
4.A potential limitation of using mRNA/cDNA display for creating proteins with desired enzymatic activity is a. Limited combinatorial diversity.
5.The sequence space of random peptides is considered small in combinatorics due to a. Limited amino acid diversity.
6.The significance of having 1.267651*10^130 possible peptides of 100 amino acids is a. It exceeds the number of atoms in the universe.
7.One might prefer a single large mutagenesis experiment on a known protein over exhaustive random peptide experiments because c. It allows for optimization of existing proteins.
8.A potential challenge in optimizing a protein obtained through a random polypeptide approach is a. Limited amino acid diversity.
9.The search for the global optimum in protein design is considered challenging due to c. Massive domain of possible proteins.
Step-by-step explanation:
1.Purpose of mRNA/cDNA display: The technique of mRNA/cDNA display is utilized in biotechnology to generate diverse libraries of random proteins for various applications, making option a. To generate random protein libraries the correct purpose of this technique.
2.Observation from ATP binding and hydrolysis: The fact that proteins selected for ATP binding also exhibited ATP hydrolysis activity suggests that the selection process can create enzymes with functional activities, making option b. It can create enzymes with functional activities the appropriate choice.
3.Use of stringent selection after initial enzymatic activity selection: More stringent selection after the initial selection for enzymatic activity might be employed to improve the initial activity, supporting option b. To improve initial activity.
4.Limitation of using mRNA/cDNA display for enzymatic activity: The technique might face a limitation due to limited combinatorial diversity, hence option a. Limited combinatorial diversity is the potential limitation.
5.Small sequence space of random peptides: The small sequence space of random peptides in combinatorics is primarily attributed to limited amino acid diversity, aligning with option a. Because of limited amino acid diversity.
6.Significance of the vast number of possible peptides: Having 1.267651*10^130 possible peptides of 100 amino acids is significant because it surpasses the number of atoms in the universe, as mentioned in option a. It exceeds the number of atoms in the universe.
7.Preference for a large mutagenesis experiment over exhaustive random peptide experiments: Conducting a single large mutagenesis experiment on a known protein over exhaustive random peptide experiments allows for the optimization of existing proteins, supporting option c. It allows for optimization of existing proteins.
8.Challenge in optimizing a protein through random polypeptide approach: A challenge in optimizing a protein obtained through a random polypeptide approach could be limited amino acid diversity, justifying option a. Limited amino acid diversity as the potential challenge.
9.Challenges in the search for global optimum in protein design: The search for the global optimum in protein design is considered challenging due to the massive domain of possible proteins, making option c. Massive domain of possible proteins the correct choice.