Question

For knocking out genes in BV-2 cell line, what supplement can be added to promote cell growth from a single cell without subcloning?

a. Conditioned media
b. Fetal bovine serum
c. Heat-inactivated FBS
d. Insulin-Transferrin-Selenium

What could be a reason for the failure of BV-2 cell subcloning and growth in DMEM media?
a. Lack of glucose
b. Excess insulin
c. Inadequate supplements
d. Presence of antibiotics

What is a potential strategy to promote cell growth from a single cell in BV-2 cell culture?
a. Subcloning in different media
b. Using conditioned media from the same culture
c. Increasing the concentration of PS in the media
d. Adding antibiotics to the culture

Answer 1

Conditioned media can be added to promote cell growth in the BV-2 cell line. Inadequate supplements may contribute to the failure of BV-2 cell subcloning and growth in DMEM media. Subcloning in different media is a potential strategy to promote cell growth from a single cell in BV-2 cell culture.

Step-by-step explanation:

To knock out genes in the BV-2 cell line and promote cell growth from a single cell without subcloning, the supplement that can be added is conditioned media. Conditioned media is the culture media that has been previously used to grow cells and contains secreted factors that promote cell growth and survival.

A potential reason for the failure of BV-2 cell subcloning and growth in DMEM media could be inadequate supplements. DMEM is a basal culture medium that may not contain all the necessary nutrients and growth factors required for optimal cell growth. Adding supplements, such as conditioned media or specific growth factors, can help promote cell growth.

A potential strategy to promote cell growth from a single cell in BV-2 cell culture is subcloning in different media. By using different types of media or supplementing the culture with conditioned media from the same culture, the cells may have a higher chance of successfully growing from a single cell.