Question

What is the potential drawback of using EGFP for transfection success detection?

a. EGFP causes excessive cell proliferation
b. Overexpression of EGFP can induce cell death
c. EGFP interferes with DNA sequencing
d. EGFP has no impact on cell viability

Why is it challenging to achieve an optimal fluorescence level for delicate embryos?
a. Delicate embryos are inherently non-fluorescent
b. Fluorescence has no impact on delicate embryos
c. Achieving optimal fluorescence may be toxic to cells
d. Delicate embryos do not require transfection

What is the primary concern with overexpression of GFP in cell cultures?
a. GFP inhibits cell differentiation
b. GFP induces unwanted mutations
c. Overexpressed GFP can cause cell death
d. GFP has no impact on cell cultures

What is an alternative method for transfection success detection without using fluorescent proteins?
a. Cell counting
b. DNA sequencing
c. Western blotting
d. PCR amplification

Why is achieving the right fluorescence level important in transgenic organisms with delicate embryos?
a. Optimal fluorescence is necessary for DNA sequencing
b. Too much fluorescence leads to cell death
c. Fluorescence has no impact on transgenic organisms
d. Achieving the right fluorescence level is irrelevant

Which method allows detection without killing organisms for sequencing in transgenic studies?
a. Fluorescence in situ hybridization (FISH)
b. Immunohistochemistry
c. PCR amplification
d. CRISPR-Cas9 gene editing

What is a potential concern when using fluorescence as an indicator of transfection success?
a. Fluorescence is always toxic to cells
b. Optimal fluorescence may not correlate with successful transfection
c. Fluorescence has no impact on cell viability
d. Achieving fluorescence is impossible in transfection studies

Answer 1

Overexpression of EGFP and GFP can lead to cell death, affecting the viability of cells and embryos in transfection experiments. Western blotting can be used as an alternative for detecting transfection success without fluorescent proteins. FISH enables detection without killing organisms in transgenic studies, and optimal fluorescence level may not always indicate successful transfection.

Step-by-step explanation:

The potential drawback of using EGFP (Enhanced Green Fluorescent Protein) for transfection success detection is that overexpression of EGFP can induce cell death. Achieving an optimal fluorescence level is challenging for delicate embryos because it may be toxic to cells, potentially leading to cell death. The primary concern with overexpression of GFP in cell cultures is similar, as overexpressed GFP can also cause cell death.

For transfection success detection without using fluorescent proteins, methods like Western blotting are an alternative. Achieving the right fluorescence level in transgenic organisms with delicate embryos is significant because it ensures the viability of the cells without causing toxicity. Fluorescence in situ hybridization (FISH) is a method that allows detection without killing organisms for sequencing in transgenic studies. When using fluorescence as an indicator of transfection success, a potential concern is that optimal fluorescence may not correlate with successful transfection, which could lead to misinterpretation of the results.