Question

Isothermal titration calorimetry (ITC) is a biophysical technique that allows a thermodynamic characterization of an interactive system. It is a free in solution technique that requires no labeling, using heat as signal. ITC allows simultaneous determination of affinity K a, stoichiometry n, enthalpy change ΔH and calculation of free energy change ΔG and entropy change ΔS in one single experiment. It is the only technique that allows direct enthalpy change measurement. By accessing the enthalpy change, we get a step closer in estimating the driving forces that characterize the interaction of a protein with a ligand, information much needed in the drug discovery process.

Answer 1

Calorimetry is a technique used to measure heat transfer in chemical or physical processes. It involves measuring temperature changes and calculating the amount of heat produced or consumed.

Step-by-step explanation:

Calorimetry is a measurement technique used to determine the amount of heat transferred in a chemical or physical process. It involves measuring temperature changes and the masses of the system and surroundings. The measurements are then used to calculate the amount of heat produced or consumed. The process is performed in a calorimeter, an insulated container that isolates the system from the surroundings. This technique is important in estimating the driving forces in protein-ligand interactions, which is useful in drug discovery.