Question

I have 1D NOESY spectra from which I need to find out the value of the NOE (%). Is it enough to simply measure the intensity of the resulting peaks or is it necessary to subtract it from the intensity of the standard spectrum?

Answer 1

To determine the value of the NOE (%) in the 1D NOESY spectra, it is necessary to subtract the intensity of the standard spectrum from the intensity of the resulting peaks.

Step-by-step explanation:

In order to determine the value of the NOE (%), it is necessary to subtract the intensity of the standard spectrum from the intensity of the resulting peaks in the 1D NOESY spectra.

The NOE (Nuclear Overhauser Effect) is a phenomenon that occurs when there is a transfer of nuclear spin polarization from one nucleus to another, resulting in an enhancement of the signal intensity in the NMR spectrum.

By subtracting the intensity of the standard spectrum, you can obtain the difference in signal intensity caused by the NOE effect, which is a measure of the NOE (%) in the sample.