Question

How can the digestion of antibodies (mainly polyclonal) by proteases during affinity chromatography be prevented?

A) Using unnatural amino acids
B) Utilizing mPEG modification
C) Both A and B
D) Using inhibitors like EDTA, Benzamidine, PMSF
E) There are no effective methods

Answer 1

To prevent the digestion of antibodies by proteases during affinity chromatography, inhibitors like EDTA, Benzamidine, and PMSF can be used.

Step-by-step explanation:

The digestion of antibodies, especially polyclonal antibodies, by proteases during affinity chromatography can be prevented using inhibitors such as EDTA, Benzamidine, and PMSF. These inhibitors can bind to the active sites of proteases, blocking their activity and preventing the digestion of the antibodies.

Using unnatural amino acids or mPEG modification would not be effective in preventing the digestion of antibodies by proteases during affinity chromatography.