Question

In the context of bacterial chromosomal transfer, how is the direction of transfer determined?

a. By the location of the origin of transfer
b. By the location of the recipient cell
c. By the orientation of rec E gene
d. By the size of the linearized chromosome

Why did the student incorrectly deduce the direction of transfer in the linearized chromosome?
a. Misinterpretation of the rec E gene location
b. Incorrect calculation of transfer time
c. Confusion between 5' and 3' ends
d. Lack of information about the recipient cell

What is the correct interpretation of the arrow pointing from the 5' end to the 3' end of the linearized chromosome?
a. Indicates the direction of chromosomal transfer
b. Represents the size of the chromosome
c. Signifies the location of the recipient cell
d. Denotes the origin of transfer

How does the transfer time of rec E compare to the overall transfer time of the linearized chromosome?
a. Rec E is one of the last genes transferred
b. Rec E is transferred simultaneously with other genes
c. Rec E is transferred before other genes
d. Transfer time of rec E is unrelated to other genes

What role does the rec E gene play in chromosomal transfer?
a. Determines the speed of transfer
b. Initiates the transfer process
c. Involved in the recognition of the recipient cell
d. Encodes information about the linearized chromosome

Answer 1

In bacterial chromosomal transfer, the direction of transfer is determined by the location of the origin of transfer. The arrow pointing from the 5' end to the 3' end of the linearized chromosome indicates the direction of chromosomal transfer. The rec E gene plays a role in the recognition of the recipient cell.

Step-by-step explanation:

In the context of bacterial chromosomal transfer, the direction of transfer is determined by the location of the origin of transfer (option a). The origin of transfer is the starting point of replication on the bacterial chromosome. The transfer of the linearized chromosome begins at the origin and progresses in a specific direction. The correct interpretation of the arrow pointing from the 5' end to the 3' end of the linearized chromosome is that it indicates the direction of chromosomal transfer (option a).

The transfer time of the rec E gene, however, is not related to the overall transfer time of the linearized chromosome. The rec E gene does not determine the speed or initiation of transfer. Instead, the rec E gene is involved in the recognition of the recipient cell (option c). It plays a role in the recombination process that allows the transferred gene to integrate into the recipient cell's chromosome. The student may have incorrectly deduced the direction of transfer in the linearized chromosome due to confusion between 5' and 3' ends (option c).

Therefore, the correct answers are: a. By the location of the origin of transfer, b. Confusion between 5' and 3' ends, a. Indicates the direction of chromosomal transfer, d. Transfer time of rec E is unrelated to other genes, c. Involved in the recognition of the recipient cell.