Question

In estimating proline content using the Ninhydrin method, what is done during the preparation of the standard curve?

A) Ninhydrin is added to the serial dilution with known proline solutions
B) The serial dilution includes a range of proline concentrations without Ninhydrin
C) Toluene is added to the serial dilution with known proline solutions
D) The standard curve is prepared without any dilution
E) The serial dilution is prepared with water only

Answer 1

In estimating proline content using the Ninhydrin method, a standard curve is prepared by adding Ninhydrin to a serial dilution of known proline solutions, with the subsequent color intensity allowing for the quantitation of proline. The correct option is A.

Step-by-step explanation:

When preparing a standard curve using the Ninhydrin method for estimating proline content, Ninhydrin is added to a serial dilution of known proline solutions.

This step is crucial since Ninhydrin reacts with proline to form a purple-blue compound, which can be measured colorimetrically.

This reaction allows for the quantitation of proline concentration based on the intensity of the color produced. Therefore, option A) Ninhydrin is added to the serial dilution with known proline solutions is the correct step taken during the preparation of the standard curve. The correct option is A.