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Is there a conventional, less expensive and accurate method for performing glucose assimilation test without using an API kit?Is it hard to get your hand on a growth medium with only a single type of carbohydrate, in another word, pure?Is in-lab contamination with other types of carbohydrates a common occurrence in conventional methods (if there any)?

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Final answer:

Yes, there are conventional methods for performing glucose assimilation tests without using an API kit. These methods include the enzymatic method and the reduction method. Obtaining a growth medium with only a single type of carbohydrate is not difficult, as there are many commercial growth media available. In-lab contamination with other types of carbohydrates is not a common occurrence in the conventional methods.

Step-by-step explanation:

Yes, there are conventional methods for performing glucose assimilation tests without using an API kit. One of the methods is the reduction method, which is based on the reducing property of glucose. This method is relatively economical and commonly used in diagnostic laboratories for estimating blood glucose levels. Another method is the enzymatic method, which uses the enzyme glucose oxidase to measure blood glucose levels. This method is more specific for glucose and provides accurate results.

It is not hard to obtain a growth medium with only a single type of carbohydrate. In fact, many commercial growth media are available that contain specific types of carbohydrates. These media can be purchased from scientific suppliers and are commonly used in microbiology laboratories.

In conventional methods of glucose assimilation testing, in-lab contamination with other types of carbohydrates is not a common occurrence. The enzymatic method and the reduction method are both specific for glucose and do not react with other carbohydrates present in the blood. However, it is important to follow proper laboratory protocols and good laboratory practices to minimize the risk of contamination.

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