Question

Can 2-mercaptoethanol be used as a substitute for 3-mercaptopropionic acid to solubilize avian eggshell membrane proteins?

a) Yes, it works equally well.
b) No, it denatures the proteins.
c) Only in the presence of a catalyst.
d) Depends on the specific proteins in the eggshell membrane.

Answer 1

2-mercaptoethanol can be used as a substitute for 3-mercaptopropionic acid to solubilize avian eggshell membrane proteins, but its effectiveness depends on the specific proteins present. Option d

Step-by-step explanation:

Avian eggshell membrane proteins can be solubilized using 3-mercaptopropionic acid, which disrupts hydrogen bonding and hydrophobic interactions in proteins.

However, 2-mercaptoethanol can also be used as a substitute, but its effectiveness depends on the specific proteins in the eggshell membrane. It is important to note that 2-mercaptoethanol and 3-mercaptopropionic acid can denature proteins if used in high concentrations or in the absence of suitable protein solubilization buffers. Option d