Question

I ran three batches of samples through the HPLC. Each batch run contained standard solutions and a set of experimental samples. The standard solutions included in each batch run were exactly the same, but the experimental samples in each batch run represented different times the experiments were run. The composition of all of the experimental samples and the standards are exactly the same. I'm not sure what is the best practice for interpreting the the unknown concentrations in the experimental samples.

(1) Individual calibration curves: Should I interpret the experimental samples associated with a batch run with a calibration curve I make from the standards of that run?

OR

(2) Consolidated calibration curve: Could I make a single calibration curve using the responses from the standards across all three batch runs to interpret the experimental samples?

Answer 1

The best practice for interpreting the unknown concentrations in the experimental samples in this case would be to use individual calibration curves made from the standards of each run.

Step-by-step explanation:

The best practice for interpreting the unknown concentrations in the experimental samples in this case would be to use individual calibration curves. You should interpret the experimental samples associated with a batch run using a calibration curve made from the standards of that run. While the composition of the experimental samples and standards across all three batch runs might be the same, it is important to consider the batch-to-batch variations that might occur during the HPLC analysis.