Final answer:
The use of masking tape as a substitute for conventional plate cover tape in shaking ELISA plates is not viable or acceptable due to the potential for evaporation, contamination, and inadequate sealing.
Step-by-step explanation:
The viability and acceptability of using masking tape as a substitute for conventional plate cover tape during the shaking of ELISA plates in hormone assays are generally questionable. ELISA (Enzyme-Linked Immunosorbent Assay) is a sensitive technique used for detecting and quantifying specific antigens, such as hormones, by using primary and secondary antibodies. During the assay, it is crucial to prevent the evaporation and cross-contamination of samples in the microtiter plates. This is typically achieved using specific plate cover tapes that are designed to maintain the integrity of the wells under various conditions, including shaking.
Masking tape, while adhesive, may not provide the same level of sealing and can lead to sample evaporation or contamination, which would compromise the assay's accuracy. Additionally, masking tape is not usually sterile and could introduce contaminants. The environment within a shaking incubator is also warm and humid, which could weaken the adhesive properties of the masking tape, leading to detachment or leaks during the assay.
In conclusion, for ELISA assays, especially when dealing with very sensitive measurements such as hormone assays, using the appropriate plate cover tape is critical for the integrity of the experiment. Therefore, the substitution of specialized plate cover tapes with makeshift solutions such as masking tape is not acceptable for maintaining the rigorous standards required in ELISA procedures.