Question

A mutation is found in eIF-2 that impairs the initiation of translation. The mutation could affect all but one of the following functions of eIF-2. Which one would not be affected?

a) Binding to the small ribosomal subunit
b) GTP hydrolysis
c) tRNA charging
d) mRNA recognition

Answer 1

The mutation in eIF-2 would not affect tRNA charging.

Step-by-step explanation:

The mutation in eIF-2 that impairs the initiation of translation would not affect tRNA charging.

eIF-2 is responsible for multiple functions in translation. It binds to the small ribosomal subunit, allows GTP hydrolysis, and is involved in mRNA recognition. These functions are essential for the initiation of translation. However, tRNA charging is a process where the correct amino acid is attached to its respective tRNA molecule, which occurs prior to translation initiation. Therefore, the mutation in eIF-2 would not impact tRNA charging.