Question

What prevents the further development of a DNA strand in Sanger sequencing?

a) DNA polymerase
b) dNTPs
c) ddNTPs (dideoxynucleotides)
d) DNA ligase

Answer 1

In Sanger sequencing, the further development of a DNA strand is prevented by the addition of ddNTPs (dideoxynucleotides).

Step-by-step explanation:

Sanger sequencing is a method used to determine the sequence of DNA. In this method, the further development of a DNA strand is prevented by the addition of ddNTPs (dideoxynucleotides), which lack a hydroxyl group (-OH) at the site where the next nucleotide would attach. When a ddNTP is incorporated into the growing DNA strand, it terminates the replication process.