Question

What are the advantages and disadvantages of SEM and TEM microscopes?

a) SEM provides 3D images, TEM provides high resolution; both have limited depth
b) TEM provides 3D images, SEM provides high resolution; both have limited depth
c) Both SEM and TEM provide 3D images with high resolution
d) Both SEM and TEM lack the ability to provide detailed images

Answer 1

The advantages of SEM include providing 3D images and not requiring thin samples, while the advantages of TEM include high resolution and the ability to view internal structures. Both SEM and TEM have a limited depth of field. SEM is useful for examining larger objects, whereas TEM is useful for viewing internal structures. However, TEM requires thin sections of specimens.

Step-by-step explanation:

The advantages and disadvantages of SEM (Scanning Electron Microscope) and TEM (Transmission Electron Microscope) microscopes are as follows:

Advantages of SEM:

• SEM provides 3D images of surfaces of specimens, allowing for detailed examination of their surface features.
• SEM doesn't require thin samples, making sample preparation easier.
• SEM is useful in examining larger objects, such as tissues and small organisms.

Disadvantages of SEM:

• SEM has a limited depth of field.
• SEM has a lower resolution compared to TEM.

Advantages of TEM:

• TEM provides high resolution and can achieve much higher magnification than light microscopes.
• TEM is useful in viewing internal structures of specimens, such as organelles and cell membranes.

Disadvantages of TEM:

• TEM also has a limited depth of field.
• TEM requires thin sections of specimens, which can be challenging to prepare.