Question

Which technique uses restriction enzyme digestion followed by agarose gel electrophoresis to generate a banding pattern for comparison to another sample processed in the same way?

a) PCR
b) DNA sequencing
c) RFLP analysis
d) Northern blotting

Answer 1

RFLP analysis is the technique that uses restriction enzyme digestion followed by agarose gel electrophoresis to generate a banding pattern for comparison to another sample processed in the same way.

Step-by-step explanation:

The technique that uses restriction enzyme digestion followed by agarose gel electrophoresis to generate a banding pattern for comparison to another sample processed in the same way is RFLP analysis (Restriction Fragment Length Polymorphism analysis).

RFLP analysis involves treating the DNA with a specific restriction endonuclease, separating the resulting fragments by size on an agarose gel, and visualizing specific fragments using complementary DNA probes.

By comparing the banding patterns of different DNA samples after restriction digestion and electrophoresis, RFLP analysis allows for the differentiation of DNA sequences and is commonly used for DNA fingerprinting and forensic analysis.