Final answer:
The object needs to be placed at -0.125 cm for the objective to produce a magnification of -400, and the eyepiece should be placed at 2.00 cm to produce a further fourfold magnification.
Step-by-step explanation:
To find the location of an object relative to a microscope for a magnification of -400 with a 0.500 cm focal length objective, we can use the magnification formula: magnification = (-di/do). Rearranging the formula, we find that the object distance (do) should be -0.125 cm. For the second part of the question, to achieve a further fourfold magnification with a 5.00 cm focal length eyepiece, we can use the equation: magnification = -di/(-do). Solving for the image distance (di), we find that it should be 2.00 cm.