Question

To make amplicons you need: Template DNA, Taq polymerase, DNA monomers, Primers, The perfect buffer solution

Options:
a) True
b) False
c) Partially true
d) None of the above

Answer 1

The statement that amplicons require template DNA, Taq polymerase, DNA monomers, Primers, and a perfect buffer solution is true. These components are essential to the PCR process, where Taq polymerase synthesizes new strands of DNA, using the template DNA and primers to amplify the desired DNA sequence.

Step-by-step explanation:

To make amplicons you do indeed need: Template DNA, Taq polymerase, DNA monomers, Primers, and The perfect buffer solution. Amplicons are DNA fragments that have been amplified using the polymerase chain reaction (PCR). In this process, the template DNA is the sequence to be copied or amplified. Taq polymerase is an enzyme that synthesizes new strands of DNA by adding nucleotides to a primer, which is bound to the template DNA. The primers are short sequences that are complementary to the ends of the target DNA sequence.

PCR also requires the four deoxynucleotide triphosphates (dNTPs) or DNA monomers which are dATP, dCTP, dGTP, and dTTP, serving as the building blocks for new DNA strand synthesis. The 'perfect buffer solution' generally includes ingredients like MgCl2, KCl, and Tris-HCl, which provide the optimal conditions for enzyme activity, DNA stability, and primer annealing. Therefore, the statement that these components are needed to create amplicons is true (option a).