Question

What technique will you use to determine the number of base pairs at your d1s80 locus?

Options:
a) Southern blotting
b) PCR amplification
c) Gel electrophoresis
d) Western blotting

Answer 1

To determine the base pair number at the d1s80 locus, PCR amplification should be used to amplify the region, followed by gel electrophoresis to separate and measure the DNA fragments.

Step-by-step explanation:

Determining the Number of Base Pairs at d1s80 Locus

To determine the number of base pairs at your d1s80 locus, the most appropriate technique to use would be PCR amplification followed by gel electrophoresis. PCR, or Polymerase Chain Reaction, is used to amplify specific regions of DNA, including the d1s80 locus. Once amplified, gel electrophoresis will separate DNA fragments based on size, allowing you to determine the length of the PCR products corresponding to different allele sizes at the d1s80 locus. This two-step process is crucial for analyzing DNA length polymorphisms, such as variable number of tandem repeats (VNTRs), which are present at the d1s80 locus.