Question

D1s80 primers will flank the d1s80 locus during PCR and provide a starting point for _____.

Options:
a) RNA synthesis
b) DNA replication
c) PCR amplification
d) Protein translation

Answer 1

The d1s80 primers are used for PCR amplification, providing a starting point for the DNA polymerase to synthesize new DNA strands.

Step-by-step explanation:

The d1s80 primers will flank the d1s80 locus during PCR and provide a starting point for PCR amplification. The role of these primers is to initiate the replication of DNA, not the synthesis of RNA or protein which excludes options a), b), and d). Primers are short sequences of DNA or RNA that are necessary for DNA polymerase to begin the synthesis of a new DNA strand. Since these primers are used in a PCR (Polymerase Chain Reaction) setup, the goal is to amplify a specific DNA region, which means making many copies of it. It is important to note that primers are essential because DNA polymerases cannot initiate the synthesis of DNA without a primer that provides a free 3'-OH end to which new nucleotides can be added.