Question

The location of which structure will you see in the cell using DAPI?

Options:
a) Golgi apparatus
b) Endoplasmic reticulum
c) Mitochondria
d) Cell membrane
e) Nucleus

Answer 1

Using DAPI, a fluorescent stain that binds to DNA, the visible structure in a eukaryotic cell would be the nucleus.

Step-by-step explanation:

When you stain a cell with DAPI, you are specifically targeting and visualizing DNA within the cell because DAPI is a fluorescent stain that binds strongly to adenine-thymine-rich regions in double-stranded DNA. Given this information, the location you would see in a cell using DAPI is the nucleus of eukaryotic cells, where the majority of the cell's genetic material is found. In prokaryotes, which lack a defined nucleus, DAPI would highlight the region where the DNA is located, usually referred to as the nucleoid.

Therefore, the correct answer to the question of which structure you would see in the cell using DAPI is e) Nucleus.