Question

What will you rotate on the fluorescence scope to capture a three-color, composite image of the FluoCells?

Options:
a) Objective lenses
b) Light source
c) Condenser
d) Stage
e) Diaphragm

Answer 1

To capture a multi-colour image of FluoCells, rotate the light source in a fluorescence microscope as it allows the excitation of different dyes for image capture in various colours.

Step-by-step explanation:

To capture a three-color, composite image of the FluoCells using a fluorescence microscope, you would rotate the light source. This is because in fluorescence microscopy, different fluorophores are excited by different wavelengths of light, and it is the light source that needs to be adjusted to alternate between these wavelengths. As the light source changes, it excites different dyes within the sample, which then emit light at different wavelengths, allowing for the capture of images in different colours that can be merged into a composite image. Therefore, while other components such as the objective lenses, condenser, stage, and diaphragm are important for sample illumination and focusing, it is the rotational adjustment of the light source that enables multicolour imaging.